Molecularly Imprinted Membrane-Based Sensor for the Detection of Chloramphenicol Succinate Residue in Milk

Molecularly imprinted films (MIF) were prepared for the determination of chloramphenicol succinate (CAP-SC) by photopolymerization method on screen printed electrodes and superficial characteristic of these films was analyzed by scanning electron microscope. A sensor based on MIF for CAP-SC detecting was assembled after the screen printed electrodes was connected with an electrochemical analyzer by an electrode slot and the detection result was recorded by a recorder connected with the electrochemical analyzer. Standard curve for CAP-SC detection was established and the determination of CAP-SC in milk samples was carried out. Scanning election microscopic images of the imprinted films shows that there was a great many of imprinted micro-pores with a diameter about 100 nm on the surface of MIF. The MIF-based sensor shows high sensitivity and specificity towards the target CAP-SC and the response of MIF-sensor to concentration of CAP-SC displayed a linear correlation over a range from 1 x 10(-8) mol/L to 1.2 x 10(-5) mol/L with a detection limit of 2 x 10(-9) mol/L. The recoveries were 93.5% - 95.5% based on milk sample.