Microfluidic integrated optoelectronic tweezers for single-cell preparation and analysis

被引：66
|
作者：
Huang, Kuo-Wei [1 ]
Wu, Yi-Chien [1 ]
Lee, Ji-Ann [2 ]
Chiou, Pei-Yu [1 ,3 ,4 ]
机构：
[1] Univ Calif Los Angeles, Dept Mech & Aerosp Engn, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Dept Biol Chem, Los Angeles, CA 90095 USA
[3] Univ Calif Los Angeles, Dept Bioengn, Los Angeles, CA 90095 USA
[4] Univ Calif Los Angeles, Calif NanoSyst Inst, Los Angeles, CA 90095 USA
来源：
LAB ON A CHIP | 2013年 / 13卷 / 18期
基金：
美国国家科学基金会;
关键词：
MANIPULATION; MICROPARTICLES;
D O I：
10.1039/c3lc50607j
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We report a novel microfluidic integrated optoelectronic tweezers (OET) platform for single-cell sample preparation and analysis. Integration of OET and microfluidics is achieved by embedding single-wall carbon nanotube (SWNT) electrodes into multilayer PDMS structures. This integrated platform allows users to selectively pick up individual cells from a population with light beams based on their optical signatures such as size, shape, and fluorescence, and transport them into isolated chambers using light induced dielectrophoretic forces. Isolated cells can be encapsulated into nanoliter liquid plugs and transported out of the platform for downstream molecule analysis using standard commercial instruments.
引用
收藏
页码：3721 / 3727
页数：7
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