Astrocytes induce several blood-brain barrier properties in non-neural endothelial cells

被引：68

作者：

Kuchler-Bopp, S ^{[1
]}

Delaunoy, JP ^{[1
]}

Artault, JC ^{[1
]}

Zaepfel, M ^{[1
]}

Dietrich, JB ^{[1
]}

机构：

[1] UPR 416 CNRS, Lab Neurobiol Mol Interact Cellulaires, F-67085 Strasbourg, France

来源：

NEUROREPORT | 1999年 / 10卷 / 06期

关键词：

astrocytes; astrocyte conditioned medium; blood-brain barrier; endothelial cell; intercellular adhesion molecule-1;

D O I：

10.1097/00001756-199904260-00035

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

THE passage of immunocompetent cells across the blood-brain barrier (BBB) is regulated at the level of the cerebral capillaries which have specific morphological and biochemical properties. We have developed and characterized an in vitro model of the BBB using immortalized human endothelial cells (ECV 304) induced by rat astrocytes. In this model, endothelial cells are attached together by continuous intercellular junctions with numerous tight junctions, develop a permeability barrier having a significant transcellular electrical resistance, possess high activities of gamma-glutamyl transpeptidase (gamma-GTP) and express the brain-type glucose transporter 1 (GLUT-1). These parameters are also characteristic of brain capillary endothelial cells. Under the culture conditions used, the ECV 304 cells express the intercellular adhesion molecule-1 (ICAM-1) on the external plasma membrane at concentrations which could permit lymphocyte adhesion to be studied. (C) 1999 Lippincott Williams & Wilkins.

引用

页码：1347 / 1353

页数：7

共 50 条

[1] Induction of various blood-brain barrier properties in non-neural endothelial cells by close apposition to co-cultured astrocytes
Hayashi, Y
Nomura, M
Yamagishi, SI
Harada, SI
Yamashita, J
Yamamoto, H
[J]. GLIA, 1997, 19 (01) : 13 - 26
[2] ASTROCYTES INDUCE BLOOD-BRAIN-BARRIER PROPERTIES IN ENDOTHELIAL-CELLS
JANZER, RC
RAFF, MC
[J]. NATURE, 1987, 325 (6101) : 253 - 257
[3] Differentiating embryonic neural progenitor cells induce blood-brain barrier properties
Weidenfeller, Christian
Svendsen, Clive N.
Shusta, Eric V.
[J]. JOURNAL OF NEUROCHEMISTRY, 2007, 101 (02) : 555 - 565
[4] Activated non-neural specific T cells open the blood-brain barrier to circulating antibodies
Westland, KW
Pollard, JD
Sander, S
Bonner, JG
Linington, C
McLeod, JG
[J]. BRAIN, 1999, 122 : 1283 - 1291
[5] Ionotropic glutamate receptors in the cells of the blood-brain barrier: Brain capillary endothelial cells and astrocytes
Balla, Z
Szilvassy, Z
Blasig, IE
[J]. GLIA, 2003, : 28 - 28
[6] Characteristics of endothelial cells derived from the blood-brain barrier and of astrocytes in culture
Ghazanfari, FA
Stewart, RR
[J]. BRAIN RESEARCH, 2001, 890 (01) : 49 - 65
[7] IN VITRO BLOOD-BRAIN BARRIER MODEL USING RAT PRIMARY BRAIN ENDOTHELIAL CELLS AND ASTROCYTES
Ci, Lei
Xiao, Guangqing
Veizaj, Elvana
Gan, Liang-Shang
[J]. DRUG METABOLISM REVIEWS, 2015, 47 : 171 - 172
[8] Astrocytes enhance radical defence in capillary endothelial cells constituting the blood-brain barrier
Schroeter, ML
Mertsch, K
Giese, H
Müller, S
Sporbert, A
Hickel, B
Blasig, IE
[J]. FEBS LETTERS, 1999, 449 (2-3) : 241 - 244
[9] Differentiation of blood-brain barrier endothelial cells
Risau, W
Esser, S
Engelhardt, B
[J]. PATHOLOGIE BIOLOGIE, 1998, 46 (03): : 171 - 175
[10] Development of blood-brain barrier endothelial cells
Risau, W
[J]. BIOLOGY AND PHYSIOLOGY OF THE BLOOD-BRAIN BARRIER: TRANSPORT, CELLULAR INTERACTIONS, AND BRAIN PATHOLOGIES, 1996, 46 : 1 - 7

← 1 2 3 4 5 →