LC-MS/MS identification of proteins from a porcine lung tissue affected by Actinobacillus pleuropneumoniae

Targeted therapy of porcine pleuropneumonia, which is caused by the bacterium Actinobacillus pleuropneumoniae, would help pig breeders to lower their expenses related with solving this problem. In order to find potential receptors of a targeted therapy it is important to know the response of the organismon an infection and detect proteins connected to an immune reaction. Nowadays, a liquid chromatography connected with tandem mass spectrometry (LC-MS/MS) is one of the most used methods for this purpose. This study was aimed at detection and identification of proteins after homogenization and extraction from the porcine lung tissue cryosections by applying LC-MS/MS method using high-performance liquid chromatography-electrospray ionisation quadrupole-quadrupole time-of-flight mass spectrometry (HPLC-ESI-QqTOF MS).