Genotypic diversity in multi-drug-resistantE. coliisolated from animal feces and Yamuna River water, India, using rep-PCR fingerprinting

被引：12

作者：

Khare, Neha ^{[1
]}

Kaushik, Megha ^{[1
]}

Martin, Juan Pedro ^{[2
]}

Mohanty, Aparajita ^{[3
]}

Gulati, Pooja ^{[1
]}

机构：

[1] Maharshi Dayanand Univ, Dept Microbiol, Med Microbiol & Bioproc Technol Lab, Rohtak, Haryana, India

[2] Univ Politecn Madrid, Escuela Tecn Super Ingn Agron Alimentaria & Biosi, Dept Biotechnol Biol Vegetal, Avda Puerta de Hierro 2-4, Madrid 28040, Spain

[3] Univ Delhi, Gargi Coll, Dept Bot, Delhi, India

来源：

ENVIRONMENTAL MONITORING AND ASSESSMENT | 2020年 / 192卷 / 11期

关键词：

E; coli; ERIC-PCR; (GTG)(5)-PCR; Molecular typing; Water management; ESCHERICHIA-COLI POPULATIONS; GENETIC DIVERSITY; FECAL POLLUTION; IDENTIFICATION; BACTERIA; DIFFERENTIATION; VALIDATION; SEQUENCES; HUMANS; INDEX;

D O I：

10.1007/s10661-020-08635-1

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Genotypic diversity among multi-drug-resistant (MDR) aquaticE. coliisolated from different sites of Yamuna River was analyzed using repetitive element PCR (rep-PCR) methods viz. ERIC-PCR and (GTG)(5)-PCR and compared with the MDR animal fecal isolates. The 97E. coliisolates belonging to different serotypes, phylogroups, and multi-drug resistance patterns were analyzed. High genetic diversity was observed by both the methods; however, (GTG)(5)typing showed higher discriminating potential. Combination of ERIC types (E1-E32) and (GTG)(5)types (G1-G46) generated 77 genotypes. The frequency of genotypes ranged from 0.013 to 0.065. The genotype composition ofE. coliisolates was highly diverse at all the sampling sites across Yamuna River except at its entry site in Delhi. The sampling sites under the influence of high anthropogenic activities showed an increase in number of unique genotype isolates. These sites also exhibited high multiple antibiotic resistance (MAR) indexes (above 0.25) suggesting high risk of contamination. Principal coordinate analysis (PCoA) showed limited clustering of genotypes based on the sampling sites. The most frequent genotypes were grouped in the positive zone of both the principal coordinates (PC1 and PC2). The genotypes of most of the animal fecal isolates were unique and occupied a common space in the negative PC1 area forming a separate cluster. High genotypic diversity among the aquaticE. coliand the drain isolates, discharging the untreated municipal waste in the river, was observed, suggesting that the sewage effluents contribute substantially to contamination of this river system than animal feces. The presence of such a high diversity among the MDRE. coliisolates in the natural river systems is of great public health significance and highlights the need of an efficient surveillance system for better management of Indian natural water bodies.

引用

页数：13