Molecular cloning and characterization of a serine/threonine protein kinase gene from Triticum aestivum

被引：0
|
作者：
Niu, JS
Yu, L
Ma, ZQ
Chen, PD [1 ]
Liu, DJ
机构：
[1] Minist Agr, Key Lab Crop Cytogenet, Nanjing 210095, Peoples R China
[2] Nanjing Agr Univ, Nanjing 210095, Peoples R China
来源：
ACTA BOTANICA SINICA | 2002年 / 44卷 / 03期
关键词：
wheat; powdery mildew; protein kinase;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To isolate genes related to resistance to Erysiphe graminis DC. ex Merat f. sp. tritici Em. Marchal in wheat (Triticum aestivum L.), differential display analysis was conducted for mRNA extracted from the seedlings of the wheat-Haynaldia villosa 6VS/6AL translocation line 92RI37 that contains the powdery mildew resistance gene Pm21. A full-length cDNA named TaPK1 was isolated. BLAST analysis revealed that it was significantly homologous to Glycine max (L.) Merr. protein kinase (GmPK6) cDNA. TaPK1 encodes a 416 amino acid long polypeptide, which belongs to serine/threonine protein kinase family, also has tyrosine kinase specificity. TaPK1 is a novel protein kinase from wheat.
引用
收藏
页码：325 / 328
页数：4
相关论文
共 50 条
  • [1] Molecular cloning and characterization of a threonine/serine protein kinase lvakt from Litopenaeus vannamei
    阮灵伟
    刘荣雕
    徐洵
    施泓
    [J]. Journal of Oceanology and Limnology, 2014, (04) : 792 - 798
  • [2] Molecular cloning and characterization of a threonine/serine protein kinase lvakt from Litopenaeus vannamei
    Lingwei Ruan
    Rongdiao Liu
    Xun Xu
    Hong Shi
    [J]. Chinese Journal of Oceanology and Limnology, 2014, 32 : 792 - 798
  • [3] Molecular cloning and characterization of a threonine/serine protein kinase lvakt from Litopenaeus vannamei
    Ruan Lingwei
    Liu Rongdiao
    Xu Xun
    Shi Hong
    [J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY, 2014, 32 (04): : 792 - 798
  • [4] Molecular cloning and characterization of a threonine/serine protein kinase lvakt from Litopenaeus vannamei
    阮灵伟
    刘荣雕
    徐洵
    施泓
    [J]. Chinese Journal of Oceanology and Limnology., 2014, 32 (04) - 798
  • [5] Cloning and characterization of a serine/threonine protein kinase from Deinococcus radiodurans
    Ghosh, S
    Grove, A
    [J]. FASEB JOURNAL, 2003, 17 (04): : A194 - A194
  • [6] Molecular cloning and biochemical characterization of a serine threonine protein kinase, PknL, from Mycobacterium tuberculosis
    Lakshminarayan, Harini
    Narayanan, Sujatha
    Bach, Horacio
    Sundaram, K. G. Papavinasa
    Av-Gay, Yossef
    [J]. PROTEIN EXPRESSION AND PURIFICATION, 2008, 58 (02) : 309 - 317
  • [7] MOLECULAR-CLONING AND CHARACTERIZATION OF A CONSERVED NUCLEAR SERINE(THREONINE) PROTEIN-KINASE
    MILLWARD, T
    CRON, P
    HEMMINGS, BA
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (11) : 5022 - 5026
  • [8] MOLECULAR-CLONING OF NOVEL HUMAN PROTEIN SERINE/THREONINE KINASE
    TENDIJKE, P
    ICHIJO, H
    FRANZEN, P
    SCHULTZ, P
    HELDIN, CH
    MIYAZONO, K
    [J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 1993, : 262 - 262
  • [9] Molecular cloning and characterization of a novel protein serine/threonine kinase highly expressed in mouse embryo
    Kurioka, K
    Nakagawa, K
    Denda, K
    Miyazawa, K
    Kitamura, N
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1998, 1443 (03): : 275 - 284
  • [10] Cloning and characterization of a novel serine/threonine protein kinase gene expressed predominantly in developing brain
    Nara, K
    Akasako, Y
    Matsuda, Y
    Fukazawa, Y
    Iwashita, S
    Kataoka, M
    Nagai, Y
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (09): : 2642 - 2651
12345