Crenarchaeota in Lake Michigan sediment

被引：172
|
作者：
MacGregor, BJ
Moser, DP
Alm, EW
Nealson, KH
Stahl, DA
机构：
[1] NORTHWESTERN UNIV,DEPT CIVIL ENGN,EVANSTON,IL 60201
[2] UNIV WISCONSIN,CTR GREAT LAKES STUDIES,MILWAUKEE,WI 53204
[3] CENT MICHIGAN UNIV,DEPT BIOL,MT PLEASANT,MI 48859
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1997年 / 63卷 / 03期
关键词：
D O I：
10.1128/AEM.63.3.1178-1181.1997
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
RNA from Lake Michigan sediment was hybridized with a DNA probe for archaeal 16S rRNA. There was a peak of archaeal rRNA abundance in the oxic zone and another immediately below it. Six contributing species were identified by PCR amplification of extracted DNA with primers specific for archaeal rDNA: two related to Methanosarcina acetivorans and four related to marine crenarchaeotal sequences. rRNA quantification using a DNA probe specific for this crenarchaeotal assemblage showed it is most abundant in the oxic zone, where it accounts for about 10% of total archaeal rRNA.
引用
收藏
页码：1178 / 1181
页数：4
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