Human iPSC-derived cardiomyocytes and tissue engineering strategies for disease modeling and drug screening

被引:100
|
作者
Smith, Alec S. T. [1 ]
Macadangdang, Jesse [1 ]
Leung, Winnie [1 ]
Laflamme, Michael A. [2 ]
Kim, Deok-Ho [1 ,3 ,4 ]
机构
[1] Univ Washington, Dept Bioengn, N410G William H Foege Bldg,3720 15th Ave NE, Seattle, WA 98195 USA
[2] Univ Hlth Network, Toronto Gen Res Inst, McEwen Ctr Regenerat Med, Toronto, ON, Canada
[3] Univ Washington, Ctr Cardiovasc Biol, Seattle, WA 98109 USA
[4] Univ Washington, Inst Stem Cell & Regenerat Med, Seattle, WA 98109 USA
关键词
Induced pluripotent stem cells; Cardiac differentiation; Tissue engineering; Disease modeling; Drug screening; PLURIPOTENT STEM-CELLS; LONG QT SYNDROME; POLYMORPHIC VENTRICULAR-TACHYCARDIA; IN-VITRO MODEL; CARDIAC TISSUE; ELECTRICAL-STIMULATION; FUNCTIONAL CARDIOMYOCYTES; DILATED CARDIOMYOPATHY; 3-DIMENSIONAL TISSUES; PRECLINICAL MODEL;
D O I
10.1016/j.biotechadv.2016.12.002
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Improved methodologies for modeling cardiac disease phenotypes and accurately screening the efficacy and toxicity of potential therapeutic compounds are actively being sought to advance drug development and improve disease modeling capabilities. To that end, much recent effort has been devoted to the development of novel engineered biomimetic cardiac tissue platforms that accurately recapitulate the structure and function of the human myocardium. Within the field of cardiac engineering, induced pluripotent stem cells (iPSCs) are an exciting tool that offer the potential to advance the current state of the art, as they are derived from somatic cells, enabling the development of personalized medical strategies and patient specific disease models. Here we review different aspects of iPSC-based cardiac engineering technologies. We highlight methods for producing iPSC-derived cardiomyocytes (iPSC-CMs) and discuss their application to compound efficacy/toxicity screening and in vitro modeling of prevalent cardiac diseases. Special attention is paid to the application of micro- and nano-engineering techniques for the development of novel iPSC-CM based platforms and their potential to advance current preclinical screening modalities. Published by Elsevier Inc.
引用
收藏
页码:77 / 94
页数:18
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