Cross-species testing of 27 pre-existing microsatellites in Podarcis gaigeae and Podarcis hispanica (Squamata: Lacertidae)

被引:7
|
作者
Runemark, Anna [1 ]
Gabirot, Marianne [2 ]
Bensch, Staffan [1 ]
Svensson, Erik I. [1 ]
Martin, Jose [2 ]
Pafilis, Panayiotis [3 ,4 ]
Valakos, Efstratios D. [5 ]
Hansson, Bengt [1 ]
机构
[1] Lund Univ, Dept Anim Ecol, SE-22362 Lund, Sweden
[2] CSIC, Museo Nacl Ciencias Nat, Dept Ecol Evolutiva, E-28006 Madrid, Spain
[3] Univ Michigan, Sch Nat Resources & Environm, Ann Arbor, MI 48109 USA
[4] Univ Michigan, Dept Class Studies, Modern Greek Program, Ann Arbor, MI 48109 USA
[5] Univ Athens, Dept Biol, Sect Anim & Human Physiol, Athens 15784, Greece
基金
瑞典研究理事会;
关键词
cross-species amplification; microsatellite; neutral molecular marker; Podarcis; population differentiation; Squamata;
D O I
10.1111/j.1755-0998.2008.02299.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We tested 27 microsatellite loci for cross-species amplification in the lacertids Podarcis gaigeae and Podarcis hispanica. We detected 11 and 15 polymorphic loci in the former and the latter species, respectively. In a larger sample of individuals from a single population of each species, the number of alleles ranged from five to 23 in 10 of the polymorphic loci in P. gaigeae, and between four and 13 in nine of polymorphic loci in P. hispanica. Two locus deviated from Hardy-Weinberg equilibrium in P. hispanica. Between 11 and 16 of the 27 loci also amplified successfully in three other Podarcis species.
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页码:1367 / 1370
页数:4
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