Erythropoietin with Retrobulbar Administration Protects Retinal Ganglion Cells from Acute Elevated Intraocular Pressure in Rats

Objective: The aim of this study was to clarify whether erythropoietin (EPO) with a retrobulbar administration could protect retinal ganglion cells (RGCs) from acute elevated intraocular pressure (IOP). Methods: The anterior chamber of the right eye was cannulated, and the IOP was raised to 70 mm Hg for a duration of up to 60 min. One thousand (1000) units of recombinant erythropoietin (rhEPO) or vehicle solution was administered a retrobulbar injection immediately after the onset of the acute elevated IOP. After I week, RGCs were labeled with a commercially available retrograde tracer applied to the superior colliculi. Densities of surviving RGCs were estimated by counting retrograde-tracer-labeled cells in whole-mounted retinas. The ultrastructural changes of RGCs were observed by transmission electron microscope. Immunocytochemistry was used to detect EPO and erythropoietin receptor (EPOR) expression in the RGCs layer. Results: The acute elevated IOP could result in the loss of RGCs. The number of surviving RGCs per square millimeter in the eyes of the acute elevated IOP + rhEPO retrobulbar injection group was significantly higher than that in the eyes of the acute elevated IOP and acute elevated IOP + vehicle solution retrobulbar injection groups (P < 0.05). The number of the organelles in the RGCs plasm decreased, but some intact mitochondrian still existed in the RGCs plasm in the eyes of the acute elevated IOP + rhEPO retrobulbar injection group. The densities of EPO and EPOR expression of the RGCs layer in the eyes of the acute elevated IOP + rhEPO retrobulbar injection group were significantly higher than that in the eyes of the acute elevated IOP and acute elevated IOP + vehicle solution retrobulbar injection groups (P < 0.01). Conclusion: EPO with a retrobulbar administration could protect RGCs from acute elevated IOP.