Sequence of Leptospira santarosai serovar Shermani genome and prediction of virulence-associated genes

被引:32
|
作者
Chou, Li-Fang [1 ,2 ]
Chen, Yu-Tin [3 ]
Lu, Chia-Wei [3 ]
Ko, Yi-Ching [1 ,2 ]
Tang, Chuan-Yi [4 ]
Pan, Ming-Jeng [5 ]
Tian, Ya-Chung [1 ,2 ]
Chiu, Cheng-Hsun [6 ]
Hung, Cheng-Chieh [1 ,2 ]
Yang, Chih-Wei [1 ,2 ]
机构
[1] Chang Gung Mem Hosp, Kidney Res Ctr, Linkou, Taiwan
[2] Chang Gung Univ, Coll Med, Gueishan, Taiwan
[3] Natl Tsing Hua Univ, Dept Comp Sci, Hsinchu 30043, Taiwan
[4] Providence Univ, Dept Comp Sci & Informat Engn, Taichung, Taiwan
[5] Cent Taiwan Univ Sci & Technol, Inst Life Sci, Taichung, Taiwan
[6] Chang Gung Mem Hosp, Mol Infect Dis Res Ctr, Linkou, Taiwan
关键词
Leptospirosis; Leptospira; Genome; Virulence; Lipoproteins; OUTER-MEMBRANE; SIGNAL PEPTIDES; PROTEIN; INSIGHTS; FEATURES; PATHOGENESIS; EXPRESSION; INFECTION;
D O I
10.1016/j.gene.2012.09.074
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Leptospirosis, a widespread zoonosis, is a re-emerging infectious disease caused by pathogenic Leptospira species. In Taiwan, Leptospira santarosai serovar Shermani is the most frequently isolated serovar, causing both renal and systemic infections. This study aimed to generate a L. santarosai serovar Shermani genome sequence and categorize its hypothetical genes, particularly those associated with virulence. The genome sequence consists of 3,936,333 nucleotides and 4033 predicted genes. Additionally, 2244 coding sequences could be placed into clusters of orthologous groups and the number of genes involving cell wall/membrane/envelope biogenesis and defense mechanisms was higher than that of other Leptospira spp. Comparative genetic analysis based on BLASTX data revealed that about 73% and 68.8% of all coding sequences have matches to pathogenic L. interrogans and L. borgpetersenii respectively, and about 57.6% to saprophyte L. biflexa. Among the hypothetical proteins, 421 have a transmembrane region, 172 have a signal peptide and 17 possess a lipoprotein signature. According to PFAM prediction, 32 hypothetical proteins have properties of toxins and surface proteins mediated bacterial attachment, suggesting they may have roles associated with virulence. The availability of the genome sequence of L. santarosai serovar Shermani and the bioinformatics re-annotation of leptospiral hypothetical proteins will facilitate further functional genomic studies to elucidate the pathogenesis of leptospirosis and develop leptospiral vaccines. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:364 / 370
页数:7
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