Site-specific cross-linking of nucleic acids using the abasic site

被引：17
|
作者：
Manoharan, M [1 ]
Andrade, LK [1 ]
Cook, PD [1 ]
机构：
[1] ISIS Pharmaceut, Dept Med Chem, Carlsbad, CA 92008 USA
来源：
ORGANIC LETTERS | 1999年 / 1卷 / 02期
关键词：
D O I：
10.1021/ol9906209
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
[GRAPHICS] An efficient site-specific cross linking reaction between two carbohydrate residues present in two complementary DNA sequences is described. One oligodeoxynucleotide, 5'd(GGCTGA*CTGCG)3', carries an amino nucleophile tethered to the 2'-hydroxyl of an adenosine residue (A*), The target electrophile is an abasic site generated in the complementary sequence, 5'd(CGCAGDCAGCC)3' (D represents the deoxyribose). The cross-linking reaction was carried out by a reductive amination reaction in >95% yield.
引用
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页码：311 / 314
页数：4
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