The Receptor Binding Domain of Botulinum Neurotoxin Serotype A (BoNT/A) Inhibits BoNT/A and BoNT/E Intoxications In Vivo

The receptor binding domain of botulinum neurotoxin (BoNT), also designated the C terminus of the heavy chain (H-C), is a promising vaccine candidate against botulism. In this study, a highly efficient expression system for the protein was developed in Escherichia coli, which provided yields that were 1 order of magnitude higher than those reported to date (350 mg H-C per liter). The product was highly immunogenic, protecting mice from a challenge with 10(5) 50% lethal dose (LD50) after a single vaccination and generating a neutralizing titer of 49.98 IU/ml after three immunizations. In addition, a single boost with H-C increased neutralizing titers by up to 1 order of magnitude in rabbits hyperimmunized against toxoid. Moreover, we demonstrate here for the first time in vivo inhibition of BoNT/A intoxication by H-C/A, presumably due to a blockade of the neurotoxin protein receptor SV2. Administration of H-C/A delayed the time to death from 10.4 to 27.3 h in mice exposed to a lethal dose of BoNT/A (P = 0.0005). Since BoNT/A and BoNT/E partially share SV2 isoforms as their protein receptors, the ability of H-C/A to cross-inhibit BoNT/E intoxication was evaluated. The administration of H-C/A together with BoNT/E led to 50% survival and significantly delayed the time to death for the nonsurviving mice (P = 0.003). Furthermore, a combination of H-C/A and a subprotective dose of antitoxin E fully protected mice against 850 mouse LD50 of BoNT/E, suggesting complementary mechanisms