Immunization via hair follicles by topical application of naked DNA to normal skin

被引：148
|
作者：
Fan, HR
Lin, Q
Morrissey, GR
Khavari, PA [1 ]
机构：
[1] Stanford Univ, Dept Mol Pharmacol, Sch Med, Stanford, CA 94305 USA
[2] Stanford Univ, Dept Dermatol, Sch Med, Stanford, CA 94305 USA
[3] VA Palo Alto Hlth Care Syst, Palo Alto, CA 94305 USA
来源：
NATURE BIOTECHNOLOGY | 1999年 / 17卷 / 09期
基金：
美国国家卫生研究院;
关键词：
gene transfer; DNA vaccine; skin; immunization; hair follicle;
D O I：
10.1038/12856
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In order to test the immune response generated to small amounts of foreign protein in skin, we applied naked DNA in aqueous solution to untreated normal skin. Topical application of plasmid expression vectors for lacZ and the hepatitis B surface antigen (HBsAg) to intact skin induced antigen-specific immune responses that displayed T-H2 features. For HBsAg, specific antibody and cellular responses were induced to the same order of magnitude as those produced by intramuscular injection of the commercially available recombinant HBsAS polypeptide vaccine. Finally, topical gene transfer was dependent on the presence of normal hair follicles.
引用
收藏
页码：870 / 872
页数：3
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