Gene Silencing Efficiency and INF-β Induction Effects of Splicing miRNA 155-Based Artificial miRNA with Pre-miRNA Stem-Loop Structures

被引：1

作者：

Sin, Onsam ^{[1
,2
,3
]}

Mabiala, Prudence ^{[1
,2
]}

Liu, Ye ^{[1
,2
]}

Sun, Ying ^{[1
,2
]}

Hu, Tao ^{[1
,2
]}

Liu, Qingzhen ^{[1
,2
]}

Guo, Deyin ^{[1
,2
]}

机构：

[1] Wuhan Univ, State Key Lab Virol, Coll Life Sci, Wuhan 430072, Peoples R China

[2] Wuhan Univ, Modern Virol Res Ctr, Coll Life Sci, Wuhan 430072, Peoples R China

[3] Kim Il Sung Univ, Coll Life Sci, Dept Biochem, Pyongyang, North Korea

来源：

BIOCHEMICAL GENETICS | 2012年 / 50卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

Interferon beta induction effect; Precursor miRNA stem-loop structure; Silencing effect; Splicing microRNA 155-based miRNA expression vector; DOUBLE-STRANDED-RNA; EXPRESSION VECTORS; PRIMARY MICRORNAS; INTERFERENCE; PATHWAYS; COMPLEX;

D O I：

10.1007/s10528-011-9476-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Artificial microRNA (miRNA) expression vectors have been developed and used for RNA interference. The secondary structure of artificial miRNA is important for RNA interference efficacy. We designed two groups of six artificial splicing miRNA 155-based miRNAs (SM155-based miRNAs) with the same target in the coding region or 3' UTR of a target gene and studied their RNA silencing efficiency and interferon beta (IFN-beta) induction effects. SM155-based miRNA with a mismatch at the +1 position and a bulge at the +11, +12 positions in a miRNA precursor stem-loop structure showed the highest gene silencing efficiency and lowest IFN-beta induction effect (increased IFN-beta mRNA level by 10% in both target cases), regardless of the specificity of the target sequence, suggesting that pSM155-based miRNA with this design could be a valuable miRNA expression vector.

引用

页码：112 / 121

页数：10