Cloning and distribution of neuropeptide W and its receptors in pigs

被引：12

作者：

Fang, Rui ^{[1
]}

Su, Juan ^{[1
]}

Zheng, Lucheng ^{[1
]}

Jin, Mengmeng ^{[1
]}

Hou, Yuanlong ^{[1
]}

Ma, Zhiyu ^{[1
]}

Guo, Tingting ^{[1
]}

Zhu, Shenzheng ^{[1
]}

Ma, Xueli ^{[1
]}

Ahmed, Ejlal ^{[1
]}

Lei, Zhihai ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China

来源：

RESEARCH IN VETERINARY SCIENCE | 2015年 / 101卷

基金：

中国国家自然科学基金;

关键词：

NPW; GPR7; GPR8; Clone; Distribution; Immunohistochemistry; PROTEIN-COUPLED RECEPTORS; PARAVENTRICULAR NUCLEUS; ENDOGENOUS LIGAND; RAT-BRAIN; GPR7; HYPOTHALAMUS; IDENTIFICATION; NEURONS; IMMUNOREACTIVITY; SECRETION;

D O I：

10.1016/j.rvsc.2015.06.001

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Neuropeptide W (NPW), a novel hypothalamic peptide, is an endogenous ligand for the orphan G proteincoupled receptors GPR7 (NPBWR1) and GPR8 (NPBWR2). Although several studies have implicated NPW in the regulation of feeding and energy metabolism in many species, the precise physiological function of NPW in pigs remains unclear. In this study, we cloned and sequenced NPW, GPR7, and GPR8 cDNA from pigs. NPW, GPR7, and GPR8 mRNA expression was quantified in the pig brain and peripheral tissues by semiquantitative reverse transcriptase polymerase chain reaction. Immunohistochemistry showed that NPW protein expression was limited in the brain and abundant in peripheral tissues. These results suggest that NPW is involved in the regulation of various physiological functions in pigs. The molecular and morphological data from this study provide a basis for further research on the functions of NPW in pigs. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：106 / 116

页数：11

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