Molecular characterization of Prototheca strains isolated from bovine mastitis

被引：21
|
作者：
Aouay, A. [1 ]
Coppee, F. [1 ]
Cloet, S. [1 ]
Cuvelier, P. [2 ]
Belayew, A. [1 ]
Lagneau, P. -E. [2 ]
Mullender, C. [2 ]
机构：
[1] Univ Mons, Mol Biol Lab, B-7000 Mons, Belgium
[2] Reg Assoc Hlth & Anim Identificat, B-5590 Ciney, Belgium
来源：
JOURNAL DE MYCOLOGIE MEDICALE | 2008年 / 18卷 / 04期
关键词：
Algae; Prototheca; Bovine; Mastitis; PCR-RFLP;
D O I：
10.1016/j.mycmed.2008.10.002
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Objective. - Prototheca zopfii are colourless algae that can cause mastitis in dairy cattle. Based on sequence analysis of the 18S rRNA gene, three genotypes are described. Material and method. - To differentiate them, a recent method of diagnostic was proposed based on rDNA amplification by Polymerase Chain Reaction (PCR) and Restriction Fragments Length Polymorphism analysis (RFLP). A total of 30 strains isolated from mastitis cases becoming of various farms were investigated. Results. - The molecular study allowed the identification of 27 strains belonging to genotype II, three to genotype III and no one to genotype I. Conclusion. - The simple PCR/RFLP system enabled the rapid, accurate and easily performed identification of genotypes of the species "zopfii". (C) 2008 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码：224 / 227
页数：4
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