Spectrofluorimetric determination of hydrogen peroxide with 2-hydroxy-1-naphthaldehyde salicyloylhydrazone (HNSH) as the substrate for horseradish peroxidase (HRP)

被引：17
|
作者：
Tang, B [1 ]
Wang, Y
Sun, Y
Shen, HX
机构：
[1] Shandong Normal Univ, Dept Chem, Jinan 250014, Peoples R China
[2] Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2002年 / 58卷 / 01期
基金：
中国国家自然科学基金;
关键词：
hydrogen peroxide determination; HNSH; HRP; catalytic spectrofluorimetry; polyethylene glycols;
D O I：
10.1016/S1386-1425(01)00514-5
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
The oxidation reaction of HNSH with H2O2 under the catalysis of HRP was studied in detail. The possible reaction mechanism was discussed. Under optimum experimental conditions, the oxidized product of HNSH had excitation and emission maxima at 296 and 414 nm, respectively. A study to prove the existence of -O-O-H in polyethylene glycols was carried out. The proposed method was successfully applied to the determination of -O-O-H in polyethylene glycols. (C) 2002 Elsevier Science B.V. All rights reserved.
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页码：141 / 148
页数：8
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