Purification and Characterization of the Acetone Carboxylase of Cupriavidus metallidurans Strain CH34

被引：13
|
作者：
Rosier, Caroline [1 ]
Leys, Natalie [2 ]
Henournont, Celine [3 ]
Mergeay, Max [2 ]
Wattiez, Ruddy [1 ]
机构：
[1] Univ Mons, Interdisciplinary Ctr Mass Spectrometry CISMa, Dept Prote & Microbiol, B-7000 Mons, Belgium
[2] SCK CEN, Expert Grp Mol & Cellular Biol, Mol, Belgium
[3] Univ Mons, NMR & Mol Imaging Lab, Dept Gen Organ & Biomed Chem, Mons, Belgium
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2012年 / 78卷 / 12期
关键词：
RHODOBACTER-CAPSULATUS; METABOLISM; PY2; FIXATION; ENZYMES;
D O I：
10.1128/AEM.07974-11
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Acetone carboxylase (Acx) is a key enzyme involved in the biodegradation of acetone by bacteria. Except for the Helicobacteraceae family, genome analyses revealed that bacteria that possess an Acx, such as Cupriavidus metallidurans strain CH34, are associated with soil. The Acx of CH34 forms the heterohexameric complex alpha(2)beta(2)gamma(2) and can carboxylate only acetone and 2-butanone in an ATP-dependent reaction to acetoacetate and 3-keto-2-methylbutyrate, respectively.
引用
收藏
页码：4516 / 4518
页数：3
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