Comparison of indirect ELISA and indirect immunofluorescence in detection of antibodies to Neospora caninum in buffaloes (Bubalus bubalis)

被引：2
|
作者：
Silva, Sandro P. [1 ]
Mota, Rinaldo A. [2 ]
Faria, Eduardo B. [2 ]
Casseb, Alexandre R. [3 ]
Casseb, Livia M. N. [4 ]
Dias, Hilma L. T. [1 ]
机构：
[1] Fed Univ Para, Inst Ciencias Biol, Lab Invest & Diagnost Enfermidades Anim, BR-66075110 Belem, Para, Brazil
[2] Univ Fed Rural Pernambuco UFRPE, Dept Vet Med, Lab Bacterioses, BR-52171900 Recife, PE, Brazil
[3] Univ Fed Rural Amazonia, Inst Saude & Prod Anim, Lab Imunol & Microbiol, BR-66077901 Belem, PA, Brazil
[4] Pesquisadora Inst Evandro Chagas, BR-67030000 Ananindeua, PA, Brazil
来源：
PESQUISA VETERINARIA BRASILEIRA | 2013年 / 33卷 / 04期
关键词：
Neospora caninum; serology; buffaloes; IFAT; ELISA; WATER-BUFFALOS; TOXOPLASMA-GONDII; CATTLE; BRAZIL; PREVALENCE; ARGENTINA; DIAGNOSIS; DOGS; MILK;
D O I：
10.1590/S0100-736X2013000400003
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
To compare two serologic tests for detection of antibodies against Neospora caninum in sera from buffaloes, samples were collected from 288 buffaloes of 2 to 10 years of age. To identify the presence of IgG, anti-N. caninum was used for the indirect immunofluorescence assay (IFAT), with title 200 as the cutoff, and for the immunoenzymatic test (indirect ELISA), considering as positive samples with ratio S/P >= 0.5. There were 153 (53.12%) animals positive for N. caninum by IFAT, whilst 50 (17.36%) animals were reactive in ELISA. The presence of antibodies anti-N. caninum demonstrates that the parasite is circulating among buffaloes raised in Para State, Brazil. ELISA and IFAT tests could be used to diagnose immunoglobulins against this agent. However there was a weak correlation (Kappa = 0.36) between both tests, considering the IFAT as the gold standard.
引用
收藏
页码：431 / 434
页数：4
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