Enhanced production of nojirimycin via Streptomyces ficellus cultivation using marine broth and inhibitory activity of the culture for seeds of parasitic weeds

被引：5

作者：

Harada, Kazuo ^{[1
]}

Kurono, Yurika ^{[1
]}

Nagasawa, Saya ^{[1
]}

Oda, Tomoka ^{[1
]}

Nasu, Yudai ^{[1
]}

Wakabayashi, Takatoshi ^{[2
]}

Sugimoto, Yukihiro ^{[3
]}

Matsuura, Hideyuki ^{[1
]}

Muranaka, Satoru ^{[4
,5
]}

Hirata, Kazumasa ^{[1
]}

Okazawa, Atsushi ^{[2
,6
]}

机构：

[1] Osaka Univ, Grad Sch Pharmaceut Sci, Appl Environm Biol, 1-6 Yamadaoka, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Grad Sch Engn, Dept Biotechnol, 2-1 Yamadaoka, Suita, Osaka 5650871, Japan

[3] Kobe Univ, Grad Sch Agr Sci, Appl Chem Biosci, Nada Ku, 1-1 Rokkodai, Kobe, Hyogo 6578501, Japan

[4] Int Inst Trop Agr, Sabo Bakin Zuwo Rd PMB 3112, Kano, Nigeria

[5] Japan Int Res Ctr Agr Sci, 1-1 Ohwashi, Tsukuba, Ibaraki 3058686, Japan

[6] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Naka Ku, 1-1 Gakuen Cho, Sakai, Osaka 5998531, Japan

来源：

JOURNAL OF PESTICIDE SCIENCE | 2017年 / 42卷 / 3-4期

关键词：

marine broth; nojirimycin; parasitic weeds; Pharmamedie (TM); Streptomyces ficellus; STRIGOLACTONES; GERMINATION; STRIGA; OROBANCHE; TARGET;

D O I：

10.1584/jpestics.D17-036

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Root parasitic weeds, such as Orobanche spp. and Striga spp., cause serious damage to crops. Recently, it was demonstrated that nojirimycin (NJ) selectively inhibits seed germination in these weeds. In this study, we modified the medium for Streptomyces ficellus to increase its production of NJ and evaluated the culture as an antiparasitic weed agent. We screened alternatives to Pharmamedia'', an additive in the original medium, and found that marine broth stimulated NJ production. Moreover, soluble starch-depleted medium could maintain S. ficellus NJ production. The NJ concentration reached 710 mg/L after four-day batch culture in starch-depleted marine broth medium, which was 17-fold higher than that in the Pharmamedia" medium. The culture in the marine broth medium inhibited seed germination of Orobanche spp. and Striga spp. as effectively as a standard solution of NJ. Pesticide Science Society of Japan

引用

页码：166 / 171

页数：6