Gene cloning and sequence analysis of cobyric acid synthase and cobalamin (5'-phosphate) synthase from hyperthermophilic archaeon Pyrococcus sp. KOD1

被引：0
|
作者：
AbdRahman, RNZ
Fujiwara, S
Imanaka, T
机构：
[1] KYOTO UNIV,GRAD SCH ENGN,DEPT SYNTHET CHEM & BIOL CHEM,SAKYO KU,KYOTO 60601,JAPAN
[2] OSAKA UNIV,GRAD SCH ENGN,DEPT BIOTECHNOL,SUITA,OSAKA 565,JAPAN
来源：
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1997年 / 83卷 / 01期
关键词：
archaea; evolution; hyperthermophile; vitamin B-12;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Two cobalamin biosynthetic genes from the archaeon Pyrococcus sp. KOD1 were cloned and sequenced. A 4.4 kb HindIII fragment from KOD1 comprised of two genes designated cobQ (1,422 bp) and cob V (696 bp) encode cobyric acid synthase (MW, 52,643 Da) and cobalamin (5'-phosphate) synthase (MW, 25,285 Da), respectively. The cob locus was identified on a chromosomal map of KOD1. The presence of cob genes in KOD1 and other methanogens suggests that the cobalamin biosynthesis pathway found in bacteria is also present in archaea.
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页码：109 / 112
页数：4
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