Radiolabeling of Umbilical Cord-Derived Mesenchymal Stem Cells for In Vivo Tracking

被引：11
|
作者：
Mukherjee, Archana
Tipnis, Shabari [3 ]
Sarma, Haladhar Dev [2 ]
Ravindran, Geeta [3 ]
Samuel, Grace [1 ]
Viswanathan, Chandra [3 ]
Venkatesh, Meera
机构：
[1] Bhabha Atom Res Ctr, Radiopharmaceut Evaluat Sect, Radiopharmaceut Div, Bombay 400085, Maharashtra, India
[2] Bhabha Atom Res Ctr, Radiat Biol & Hlth Sci Div, Bombay 400085, Maharashtra, India
[3] Reliance Life Sci Pvt Ltd, Regenerat Med, DALC, Rabale, Navi Mumbai, India
来源：
CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS | 2012年 / 27卷 / 09期
关键词：
Cr-51-labeled stem cells; radiolabeled stem cells; umbilical cord mesenchymal stem cells (UCMSC); ORGAN DISTRIBUTION;
D O I：
10.1089/cbr.2011.1146
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Non-invasive methods for the assessment of distribution, homing, and retention of stem cells are desired for the successful demonstration of stem cell therapy. Cells labeled with Tc-99m, F-18, and In-111 have been reported for tracking the cells in vivo. However, they can be tracked only for a limited time due to the short half lives of these isotopes. In this context, stem cells labeled with Cr-51 would be appropriate for tracking cells for a longer period of time owing to their half life of 27.7 days. Here, we have isolated mesenchymal stem cells (MSCs) from umbilical cord tissue, characterized them, and attempted to radiolabel them with Cr-51 for mapping the fate of transplanted MSC cells after an intravenous injection via the tail vein in small animals.
引用
收藏
页码：614 / 619
页数：6
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