A 5′-nuclease real-time reverse transcriptase-polymerase chain reaction assay for the detection of a broad range of influenza A subtypes, including H5N1

被引：36
|
作者：
Whiley, DM [1 ]
Sloots, TP
机构：
[1] Royal Childrens Hosp & Hlth Serv Dist, Sir Albert Sakzewski Virus Res Ctr, Clin Virol Res Unit, Herston, Qld 4029, Australia
[2] Univ Queensland, Clin Med Virol Ctr, Brisbane, Qld 4072, Australia
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2005年 / 53卷 / 04期
关键词：
influenza; PCR; real-time; H5N1;
D O I：
10.1016/j.diagmicrobio.2005.08.002
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
A 5'-nuclease real-time reverse transcriptase-polymerase chain reaction assay was developed for the detection of influenza type A and was validated using a range of influenza A subtypes, including avian strains, and 126 nasopharyngeal aspirate samples. The results show the assay is suitable for screening for influenza A infections, particularly in regions where avian strains may be circulating. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码：335 / 337
页数：3
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