Relative expression profile of lingual antimicrobial peptide (LAMP) mRNA by different tissues of Bubalus bubalis

Lingual antimicrobial peptide (LAP) is a member of the beta-defensin family and predominantly expressed by the tongue epithelium. Present study was conducted to investigate the relative expression of LAP mRNA by different tissues of Bubalus bubalis. Different tissues, viz., tongue, testis, uterus, liver and myeloids from bone were collected from freshly slaughtered healthy animal. Total RNA was extracted and cDNA was synthesized. Relative expression of LAP mRNA by different tissues was studied using real-time PCR with reference to buffalo beta-actin gene. Cycle threshold (Ct) values were determined and Delta Ct value for each tissue was estimated. The tongue epithelia showed the highest level of LAP gene expression (Delta Ct -11.46), followed by uterus (Delta Ct -11.27), testis (Delta Ct -8.16), liver (Delta Ct -4.89) and myeloid tissues (Delta Ct 0.10). From the present study it can be concluded that B. bubalis LAP is constitutively expressed by different tissues.