Enzyme immobilization technique in microfluidic channel on integrated chip

A new method for the immobilization of enzyme in microfluidic channel on the integrated chip for proteomic analysis was reported. The PDMS film immersing in the acrylic acid solution was irradiated under UV lamp to produce active free radicals and then immersed alternatively into the PDDA solution and trypsin solution for self-assemble. The monomer solution of the produced chip was characterized with the reflectance IR technique, contact angle determination and SEM observations. A standard protein BSA was selected to test the performance of the product. It was verified that CE and MALDI-TOF/TOF-MS detection can be on-line carried out in a single, successive running.