Proliferation and osteoblastic differentiation of hMSCs on cellulose-based hydrogels

被引：30
|
作者：
Raucci, Maria Grazia [1 ]
Alvarez-Perez, Marco Antonio [1 ]
Demitri, Christian [2 ]
Sannino, Alessandro [2 ]
Ambrosio, Luigi [1 ]
机构：
[1] Natl Res Council Italy, Inst Composite & Biomed Mat, I-80125 Naples, Italy
[2] Univ Salento, Dept Engn Innovat, Lecce, Italy
来源：
JOURNAL OF APPLIED BIOMATERIALS & FUNCTIONAL MATERIALS | 2012年 / 10卷 / 03期
关键词：
Biocompatibility; Cellulose; Injectable gel; Mesenchymal stem cells; CELL-ADHESION; FUNCTIONAL-GROUPS; WETTABILITY; BIOMATERIALS; PHOSPHATES; ADSORPTION; SCAFFOLDS; SURFACES; MATRICES; GROWTH;
D O I：
10.5301/JABFM.2012.10366
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Purpose: The aim of this project was to study the proliferation and differentiation of human Mesenchymal Stem Cells (hMSCs) onto a cellulose-based hydrogel for bone tissue engineering. Methods: Modified-cellulose hydrogel was prepared via double esterification crosslinking using citric acid. The response of hMSCs in terms of cell proliferation and differentiation into osteoblastic phenotype was evaluated by using Alamar blue (TM) assay and alkaline phosphatase (ALP) activity. Results: The results showed that CMCNa and CMCNa_CA have no negative effect on hMSC, adhesion and proliferation. Moreover, the increase of the ALP expression for CMCNa_CA confirms the ability of the hydrogels to support the osteoblastic differentiation. Conclusions: The cellulose-based hydrogels have a potential application as filler in bone tissue regeneration.
引用
收藏
页码：302 / 307
页数：6
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