Chronic consumption of quercetin reduces erythrocytes oxidative damage: Evaluation at resting and after eccentric exercise in humans

被引:34
|
作者
Duranti, Guglielmo [1 ]
Ceci, Roberta [1 ]
Patrizio, Federica [3 ]
Sgro, Paolo [2 ]
Di Luigi, Luigi [2 ]
Sabatini, Stefania [1 ]
Felici, Francesco [3 ]
Bazzucchi, Ilenia [3 ]
机构
[1] Univ Roma Foro Italico, Dept Movement Human & Hlth Sci, Unit Biol Genet & Biochem, Rome, Italy
[2] Univ Roma Foro Italico, Dept Movement Human & Hlth Sci, Unit Endocrinol, Rome, Italy
[3] Univ Roma Foro Italico, Dept Movement Human & Hlth Sci, Lab Exercise Physiol, Rome, Italy
关键词
Quercetin; Human erythrocytes; ex vivo hemolysis; in vivo redox homeostasis; Eccentric exercise; DIETARY FLAVONOID QUERCETIN; SKELETAL-MUSCLE; IN-VITRO; ANTIOXIDANT ACTIVITY; EX-VIVO; STRESS; BLOOD; SUPPLEMENTATION; HEMOLYSIS; TADALAFIL;
D O I
10.1016/j.nutres.2017.12.002
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
The polyphenolic flavonoid quercetin has been shown to be a powerful antioxidant, in vitro and in murine models. However, its effect on redox status has been poorly examined in humans, particularly in combination with strenuous exercise. We hypothesized that quercetin supplementation would beneficially affect redox homeostasis in healthy individuals undergoing eccentric exercise. To test this hypothesis, the effects of chronic consumption of quercetin on glutathione system (reduced, oxidized, and reduced to oxidized glutathione ratio), oxidative damage (thiobarbituric acid reactive substances (TBARs)], antioxidant enzymatic network (catalase, glutathione peroxidase, superoxide dismutase) and resistance to lysis, were investigated in erythrocytes, a traditional model widely used to study the effects of oxidative stress as well as the protective effects of antioxidants. In a two weeks controlled, randomized, crossover, intervention trial, 14 individuals ingested 2 caps (1 g/d) of quercetin or placebo. Blood samples were collected before, after 2 weeks of supplementation and after a bout of eccentric exercise. Quercetin, reduced significantly erythrocytes lipid peroxidation levels and the susceptibility to hemolysis induced by the free radical generator AAPH, while no differences in antioxidant enzyme activities and glutathione homeostasis were found between the two groups. After a single bout of eccentric exercise, quercetin supplementation improved redox status as assessed by reduced/oxidized glutathione ratio analysis and reduced TBARs levels both in erythrocytes and plasma. In conclusion, our study provides evidences that chronic quercetin supplementation has antioxidant potential prior to and after a strenuous eccentric exercise thus making the erythrocytes capable to better cope with an oxidative insult. (C) 2017 Elsevier Inc. All rights reserved.
引用
收藏
页码:73 / 81
页数:9
相关论文
共 23 条
  • [1] Quercetin supplementation decreases erythrocytes oxidative damage at resting and after an acute bout of eccentric exercise in humans
    Duranti, Guglielmo
    Ceci, Roberta
    Patrizio, Federica
    Bazzucchi, Ilenia
    Sgro, Paolo
    Di Luigi, Luigi
    Sabatini, Stefania
    Felici, Francesco
    [J]. FREE RADICAL BIOLOGY AND MEDICINE, 2017, 108 : S88 - S89
  • [2] Effects of Quercetin Supplementation on Markers of Muscle Damage and Inflammation After Eccentric Exercise
    O'Fallon, Kevin S.
    Kaushik, Diksha
    Michniak-Kohn, Bozena
    Dunne, C. Patrick
    Zambraski, Edward J.
    Clarkson, Priscilla M.
    [J]. INTERNATIONAL JOURNAL OF SPORT NUTRITION AND EXERCISE METABOLISM, 2012, 22 (06) : 430 - 437
  • [3] Motor unit activity after eccentric exercise and muscle damage in humans
    Semmler, J. G.
    [J]. ACTA PHYSIOLOGICA, 2014, 210 (04) : 754 - 767
  • [4] Chronic quercetin ingestion and exercise-induced oxidative damage and inflammation
    McAnulty, Steven R.
    McAnulty, Lisa S.
    Nieman, David C.
    Quindry, John C.
    Hosick, Peter A.
    Hudson, Matthew H.
    Still, Laura
    Henson, Dru A.
    Milne, Ginger L.
    Morrow, Jason D.
    Dumke, Charles L.
    Utter, Alan C.
    Triplett, Nan T.
    Dibarnardi, Adrianna
    [J]. APPLIED PHYSIOLOGY NUTRITION AND METABOLISM, 2008, 33 (02) : 254 - 262
  • [5] Low level laser therapy before eccentric exercise reduces muscle damage markers in humans
    Baroni, Bruno Manfredini
    Pinto Leal Junior, Ernesto Cesar
    De Marchi, Thiago
    Lopes, Andre Luiz
    Salvador, Mirian
    Vaz, Marco Aurelio
    [J]. EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, 2010, 110 (04) : 789 - 796
  • [6] Low level laser therapy before eccentric exercise reduces muscle damage markers in humans
    Bruno Manfredini Baroni
    Ernesto Cesar Pinto Leal Junior
    Thiago De Marchi
    André Luiz Lopes
    Mirian Salvador
    Marco Aurélio Vaz
    [J]. European Journal of Applied Physiology, 2010, 110 : 789 - 796
  • [7] Resistance-Trained Individuals Are Less Susceptible to Oxidative Damage after Eccentric Exercise
    Spanidis, Ypatios
    Stagos, Dimitrios
    Papanikolaou, Christina
    Karatza, Konstantina
    Theodosi, Andria
    Veskoukis, Aristidis S.
    Deli, Chariklia K.
    Poulios, Athanasios
    Koulocheri, Sofia D.
    Jamurtas, Athanasios Z.
    Haroutounian, Serkos A.
    Kouretas, Demetrios
    [J]. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2018, 2018
  • [8] N-acetylcysteine supplementation and oxidative damage and inflammatory response after eccentric exercise
    Silva, Luciano A.
    Silveira, Paulo C. L.
    Pinho, Cleber A.
    Tuon, Talita
    Dal Pizzol, Felipe
    Pinho, Ricardo A.
    [J]. INTERNATIONAL JOURNAL OF SPORT NUTRITION AND EXERCISE METABOLISM, 2008, 18 (04) : 379 - 388
  • [9] Strength Loss After Eccentric Exercise Is Related to Oxidative Stress but Not Muscle Damage Biomarkers
    Cakir-Atabek, Hayriye
    Dokumaci, Bircan
    Aygun, Cihan
    [J]. RESEARCH QUARTERLY FOR EXERCISE AND SPORT, 2019, 90 (03) : 385 - 394
  • [10] Fish Oil Supplementation Reduces Markers of Oxidative Stress But Not Muscle Soreness After Eccentric Exercise
    Gray, Patrick
    Chappell, Andrew
    Jenkinson, Alison McE
    Thies, Frank
    Gray, Stuart R.
    [J]. INTERNATIONAL JOURNAL OF SPORT NUTRITION AND EXERCISE METABOLISM, 2014, 24 (02) : 206 - 214