N-Terminal Labeling of Peptides by Trypsin-Catalyzed Ligation for Quantitative Proteomics

被引：19
|
作者：
Pan, Yanbo [1 ,2 ]
Ye, Mingliang [1 ]
Zhao, Liang [1 ]
Cheng, Kai [1 ,2 ]
Dong, Mingming [1 ,2 ]
Song, Chunxia [1 ,2 ]
Qin, Hongqiang [1 ,2 ]
Wang, Fangjun [1 ]
Zou, Hanfa [1 ]
机构：
[1] Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 35期
关键词：
enzyme catalysis; isotopic labeling; peptides; quantitative proteomics; terminal labeling; MASS-SPECTROMETRY; SENSITIVITY;
D O I：
10.1002/anie.201303429
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Two times an enzyme: Trypsin was first used as a protease to catalyze the digestion of proteins and then as a ligase to catalyze the linking of isotopically labeled amino acids to the Ntermini of tryptic peptides for quantitative proteomics. Reliable and accurate quantification of proteins was demonstrated for model proteins as well as proteome samples. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：9205 / 9209
页数：5
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