The Effect of Biofuels on Colony Formation of CHO-9 Cells

The four types of biofuels obtained by methanol transesterification of: rapeseed expired oil (BP I), waste animal fats (BP II), frying vegetable oils (BP III), and rapeseed oil (BP IV) were studied on the Chinese hamster ovary (CHO-9) cells using the clonogenic assay. Colony test results showed that tested biofuels inhibited the ability of CHO-9 cells to form colonies, depending on the type and concentration of the biofuel being tested. The strongest action in reducing CHO-9 cell proliferation capacity was demonstrated by biofuel II and biofuel III. Biofuel IV had the least effect on the ability of the CHO-9 cells to colonize. The results suggest that biofuels might play a role in molecular mechanisms associated with cellular proliferation and immune responses, that should be confirmed in the future studies on genotoxicity of biofuels.