Gelatin-based membrane containing usnic acid-loaded liposomes: A new treatment strategy for corneal healing

被引：16
|
作者：
da Silva, Flavia Rodrigues [1 ,2 ]
Silva, Rummenigge Oliveira [2 ]
de Castro Oliveira, Hortencia Maciel [3 ]
Nunes Dourado, Lays Fernanda [2 ]
da Costa, Bruna Lopes [2 ]
Lima, Bruno Santos [4 ]
Lima Santos, Nayara Gomes [5 ]
Palanch Repeke, Carlos Eduardo [1 ]
Menezes, Gustavo Batista [3 ]
de Souza Araujo, Adriano Antunes [4 ,5 ]
da Silva-Cunha, Armando [2 ]
Nunes, Paula Santos [1 ,5 ]
机构：
[1] Univ Fed Sergipe, Programa Posgrad Ciencias Aplicadas Saude, BR-49400000 Lagarto, SE, Brazil
[2] Univ Fed Minas Gerais, Fac Farm, BR-31270901 Belo Horizonte, MG, Brazil
[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Morfol, BR-31270010 Belo Horizonte, MG, Brazil
[4] Univ Fed Sergipe, Programa Posgrad Ciencias Farmaceut, BR-49100100 Lagarto, SE, Brazil
[5] Univ Fed Sergipe, Programa Posgrad Ciencias Saude, BR-49100100 Lagarto, SE, Brazil
来源：
BIOMEDICINE & PHARMACOTHERAPY | 2020年 / 130卷
基金：
巴西圣保罗研究基金会;
关键词：
Usnic acid; Eye burn; Healing; ENDOTHELIAL GROWTH-FACTOR; CHICK CHORIOALLANTOIC MEMBRANE; OPHTHALMIC DRUG-DELIVERY; CELLULAR MECHANISMS; ANGIOGENESIS; SYSTEM; NEOVASCULARIZATION; ORGANIZATION; BEVACIZUMAB; EXPRESSION;
D O I：
10.1016/j.biopha.2020.110391
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Purpose: To evaluate the safety and potential healing efficacy of the topical ocular administration of a gelatin membrane containing usnic acid/liposomes (UALs) for corneal cicatrization. UALs have shown healing activity in animal models of dermal burn lesions. We evaluated the safety of topical ocular administration of UAL and its potential healing efficacy as an ophthalmic treatment on chemical lesions in rabbit eyes. Method: The Draize test was used to check for ocular toxicity and the score was zero at each observation, indicating the ocular safety of a gelatin membrane containing usnic acid/liposome. Its potential healing efficacy as an ophthalmic treatment on chemical lesions in rabbit eyes was also assessed. Results: After epithelial removal and treatment with UAL, there was a 49.4 % reduction in injury under in vivo conditions compared with a 36.6 % reduction in the control, a gelatin membrane containing liposome without usnic acid. Histological analysis of ocular surface chemical injury-tissue sections after treatment with UAL supported these observations. The corneal expression of VEGF and TGF-beta 1increased by 70 % and 50 % respectively following treatment with UAL gelatin membrane. Conclusion: These results indicate the potential therapeutic application of UAL gelatin membranes as an ophthalmic treatment that may be used for corneal cicatrization.
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页数：12
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