A simple method for efficient separation and purification of c-phycocyanin and allophycocyanin from Spirulina platensis

被引：79
|
作者：
Zhang, YM
Chen, F [1 ]
机构：
[1] Univ Hong Kong, Dept Bot, Pokfulam Rd, Hong Kong, Peoples R China
[2] Guizhou Univ Technol, Light Ind Dept, Guiyang, Peoples R China
来源：
BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 09期
关键词：
allophycocyanin; c-phycocyanin; purification; separation; Spirulina platensis;
D O I：
10.1023/A:1008914405302
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
c-Phycocyanin and allophycocyanin were separated and purified from Spirulina platensis by precipitation with ammonium sulphate, ion exchange chromatography and gel filtration chromatography. Pure c-phycocyanin and allophycocyanin were finally obtained with an A(620)/A(280)value of 5.06 and an A(655)/A(280) value of 5.34, respectively.
引用
收藏
页码：601 / 603
页数：3
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