Gliadin and glutenin signal genes of spelt (Triticum spelta L)

Analysis of six breeders' lines of spelt (Triticum spelta L.) of the German origin was carried out using signal genes which determined gliadins and the high molecular weight (HMW) glutenins of wheat grain. Electrophoretic spectra of gliadins were obtained by the method SGE (Sasek, Sykorova, 1989); high molecular weight (HMW) glutenins by using the method SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis; Laemmli, 1970). Allelic blocks of gliadin zones were separated according to the procedure of Sobko, Poperelja (1986), allelic blocks of zones of the HMW glutenins according to Payne et al. (1981, 1987, 1988). In electrophoretic structure of gliadins new allelic blocks GLD 1-1A N3 and GLD 6B N11 were found, not classified till now. According to the occurrence of allelic blocks of gliadins and HMW glutenins - markers of baking quality, frost hardiness and resistance to powdery mildew of cereals and grasses - potential agronomic value of tested new breeders' lines of spelt was assessed. Indices of identity of sets of allelic blocks of gliadins and HMW glutenins were used to evaluate the relation of new breeders' lines of spelt tested.