Comparison of direct fecal smear microscopy, culture, and polymerase chain reaction for the detection of Blastocystis sp in human stool samples

被引:25
|
作者
Santos, Herbert J. [1 ]
Rivera, Windell L. [1 ,2 ]
机构
[1] Univ Philippines, Coll Sci, Inst Biol, Quezon City 1101, Philippines
[2] Univ Philippines, Nat Sci Res Inst, Mol Protozool Lab, Quezon City 1101, Philippines
关键词
Blastocystis sp; Direct fecal smear; Culture; Polymerase chain reaction (PCR); Human stool; Sensitivity; Specificity; IN-VITRO CULTIVATION; MOLECULAR CHARACTERIZATION; HOMINIS; PCR; EXTRACTION; DNA;
D O I
10.1016/S1995-7645(13)60138-8
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Objective: To compare the sensitivity and specificity of direct fecal smear microscopy, culture, and polymerase chain reaction in the detection of Blastocystis sp. in human stool. Methods: Human stool samples were collected from a community in San Isidro, Rodriguez, Rizal, Philippines. These samples were subjected to direct fecal smear microscopy, culture and polymerase chain reaction to detect the presence of Blastocystis sp. Results: Of the 110 stool samples collected, 28 (25%) were detected positive for the presence of Blastocystis sp. by two or more tests. Culture method detected the highest number of Blastocystis positive stool samples (n=36), followed by PCR of DNA extracted from culture (n=26), PCR of DNA extracted from stool (n=10), and direct fecal smear (n=9). Compared to culture, the sensitivity of the other detection methods were 66.7% for PCR from culture and 19.4% for both PCR from stool and direct fecal, smear. Specificity of the methods was high, with PCR from culture and direct fecal smear having 97.3%, while PCR from stool at 95.9%. Conclusions: In this study, in vitro culture is the best method for detecting Blastocystis sp. in human stool samples.
引用
收藏
页码:780 / 784
页数:5
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