First genome survey and repeatome analysis ofChrysopogon zizanioidesbased on next-generation sequencing

Chrysopogon zizanioidesis a perennial C4 grass that has been widely utilized for soil erosion control and ecological restoration. Nevertheless, genomic information ofC. zizanioidesis currently unavailable. Here, we firstly conducted a genome survey ofC. zizanioidesto obtain its repeatome information through next generation sequencing (NGS) and RepeatExplorer analysis. As a result, the estimated genome size ofC. zizanioideswas about 880 Mb, the heterozygosity rate was calculated to be 0.80%, the repeat content was 62.08%, and the GC content was 44.71%. Nine different repeat families were identified in theC. zizanioidesgenome, Ty3/Gypsy retrotransposons represented the dominant family and demonstrated greater diversity than Ty1/Copia retrotransposons, and twoC. zizanioidesrDNAs displayed high conservation in the genic coding regions and high polymorphism in the intergenic spacer regions. Overall, these results enhanced our understanding of theC. zizanioidesgenome, and provided a large amount of genomic resources for further excavation and utilization ofC. zizanioides.