State transitions and nonphotochemical quenching during a nutrient-induced fluorescence transient in phosphorus-starved Dunaliella tertiolecta

被引：23

作者：

Petrou, K. ^{[2
,3
]}

Doblin, M. A. ^{[2
,3
]}

Smith, R. A. ^{[2
,3
]}

Ralph, P. J. ^{[2
,3
]}

Shelly, K. ^{[1
]}

Beardall, J. ^{[1
]}

机构：

[1] Monash Univ, Sch Biol Sci, Clayton, Vic 3800, Australia

[2] Univ Technol Sydney, Inst Water & Environm Resource Management, Sydney, NSW 2007, Australia

[3] Univ Technol Sydney, Dept Environm Sci, Sydney, NSW 2007, Australia

来源：

JOURNAL OF PHYCOLOGY | 2008年 / 44卷 / 05期

基金：

澳大利亚研究理事会;

关键词：

77 K fluorescence; Dunaliella tertiolecta; NIFTs; nonphotochemical quenching; phosphorus starvation; state transitions;

D O I：

10.1111/j.1529-8817.2008.00585.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Assessments of nutrient-limitation in microalgae using chl a fluorescence have revealed that nitrogen and phosphorus depletion can be detected as a change in chl a fluorescence signal when nutrient-starved algae are resupplied with the limiting nutrient. This photokinetic phenomenon is known as a nutrient-induced fluorescence transient, or NIFT. Cultures of the unicellular marine chlorophyte Dunaliella tertiolecta Butcher were grown under phosphate starvation to investigate the photophysiological mechanism behind the NIFT response. A combination of low temperature (77 K) fluorescence, photosynthetic inhibitors, and nonphotochemical quenching analyses were used to determine that the NIFT response is associated with changes in energy distribution between PSI and PSII and light-stress-induced nonphotochemical quenching (NPQ). Previous studies point to state transitions as the likely mechanism behind the NIFT response; however, our results show that state transitions are not solely responsible for this phenomenon. This study shows that an interaction of at least two physiological processes is involved in the rapid quenching of chl a fluorescence observed in P-starved D. tertiolecta: (1) state transitions to provide the nutrient-deficient cell with metabolic energy for inorganic phosphate (P-i)-uptake and (2) energy-dependent quenching to allow the nutrient-stressed cell to avoid photodamage from excess light energy during nutrient uptake.

引用

页码：1204 / 1211

页数：8