Use of soft X-ray microscopy for analysis of early-stage biofilm formation

被引：11
|
作者：
Gilbert, ES [1 ]
Khlebnikov, A
Meyer-Ilse, W
Keasling, JD
机构：
[1] Univ Calif Berkeley, Dept Chem Engn, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Lawrence Berkeley Lab, Ctr Xray Opt, Berkeley, CA 94720 USA
来源：
WATER SCIENCE AND TECHNOLOGY | 1999年 / 39卷 / 07期
关键词：
microscopy; imaging; colonization; spatial distribution; biofilm structure;
D O I：
10.2166/wst.1999.0368
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Soft X-ray microscopy (SXM) using synchrotron radiation is a newly-available technology for high resolution imaging of hydrated specimens, making it potentially valuable for the study of biofilms. In this research, SXM was used to investigate bacterial spatial distribution and biofilm structure during the early stages of colonization of an exposed surface. Pseudomonas putida DMP-1 formed a closely-packed monolayer on a silicon nitride substratum after 24 hours of growth. The initial development of a multilayer, interlocking structure was observed. The soft X-ray images were taken with the XM-1 transmission X-ray microscope, located at the Center for X-ray Optics, Lawrence Berkeley National Laboratory. The results indicate the potential of SXM to provide new insights for the study of biofilms. (C) 1999 IAWQ Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：269 / 272
页数：4
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