Pros and Cons of In Vitro Methods for Circular RNA Preparation

被引：19
|
作者：
Lee, Kyung Hyun [1 ]
Kim, Seongcheol [1 ]
Lee, Seong-Wook [1 ,2 ]
机构：
[1] Rznomics Inc, R&D Ctr, Seongnam 13486, South Korea
[2] Dankook Univ, Res Inst Adv Omics, Dept Bioconvergence Engn, Yongin 16890, South Korea
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 21期
基金：
新加坡国家研究基金会;
关键词：
circular RNA; circularization; RNA; RNA vaccine; RNA therapeutics; ENHANCES TRANSLATION; CYANOGEN-BROMIDE; DNA DUPLEXES; SELF; LIGATION; INTRON; MECHANISMS; DESIGN; LIGASE; STRAND;
D O I：
10.3390/ijms232113247
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
mRNA is gaining success as a new therapeutic agent and vaccine. However, mRNA has limitations in stability. To overcome the shortcomings of mRNA, circular RNA is emerging as a new modality. In this review, several current methods of manufacturing circular RNA in vitro are introduced and their advantages and disadvantages are reviewed. Furthermore, this study discusses which fields and directions of research and development are needed for the increase in the efficacy and productivity of circular RNA as a therapeutic agent and vaccine formulation.
引用
收藏
页数：10
相关论文
共 50 条
  • [1] The Pros and Cons of Circular RNAs as miRNA Sponges
    Duk M.A.
    Samsonova M.G.
    Biophysics, 2021, 66 (1) : 8 - 16
  • [2] Pros and cons of methods of prolapse surgery
    Van Geelen, J. M.
    22ND EUROPEAN CONGRESS OF OBSTETRICS AND GYNAECOLOGY (EBCOG 2012), 2012, : 59 - 66
  • [3] The Pros and Cons of the PROs
    Thoma, Achilleas
    Eaves, Felmont F., III
    AESTHETIC SURGERY JOURNAL, 2018, 38 (03) : 343 - 345
  • [4] An update on in vitro test methods in human hepatic drug biotransformation research: pros and cons
    Brandon, EFA
    Raap, CD
    Meijerman, I
    Beijnen, JH
    Schellens, JHM
    TOXICOLOGY AND APPLIED PHARMACOLOGY, 2003, 189 (03) : 233 - 246
  • [5] IMPROVING SAT SCORES - PROS, CONS, METHODS
    BRAZZIEL, WF
    JOURNAL OF NEGRO EDUCATION, 1988, 57 (01): : 81 - 93
  • [6] The pros and cons of various pallet wrapping methods
    Thysen, A
    ZKG INTERNATIONAL, 2003, 56 (11): : 35 - 40
  • [7] PROS AND CONS
    不详
    LANCET, 1949, 257 (SEP3): : 440 - 440
  • [8] THE PROS OF CONS
    Chant, Ian
    LIBRARY JOURNAL, 2016, 141 (12) : 26 - 29
  • [9] Pros cons
    Dean, B
    STRENGTH AND CONDITIONING JOURNAL, 2002, 24 (02) : 53 - 54
  • [10] THE PROS AND THE CONS
    BERNSTEIN, PL
    JOURNAL OF PORTFOLIO MANAGEMENT, 1986, 12 (02): : 1 - 1
12345