Surface Plasmon Resonance Studies on Molecularly Imprinted Films

被引：12
|
作者：
Yoshikawa, Masakazu [1 ]
Guiver, Michael D. [2 ]
Robertson, Gilles P. [2 ]
机构：
[1] Kyoto Inst Technol, Dept Biomol Engn, Kyoto 6068585, Japan
[2] Natl Res Council Canada, Inst Chem Proc & Environm Technol, Ottawa, ON K1A 0R6, Canada
来源：
JOURNAL OF APPLIED POLYMER SCIENCE | 2008年 / 110卷 / 05期
关键词：
adenosine; guanosine; 9-ethyladenine; molecular imprinting; molecular recognition; polysulfone; surface plasmon resonance;
D O I：
10.1002/app.28686
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
Novel molecular recognition films were prepared from modified polysulfone having perillaldehyde moiety as a side group. The molecular recognition films were obtained from perillaldehyde polysulfone by adopting 9-ethyladenine as a print molecule. The molecular recognition phenomena were studied by surface plasmon resonance (SPR) spectroscopy. Adsorption of adenosine (As) and guanosine (Gs) in the molecularly imprinted film was studied. Dual adsorption isotherms were observed for As in 9-EA imprinted films, while nonspecific adsorption isotherms for Gs in those films. This revealed that the molecular recognition sites toward As were constructed in the films thus prepared. The apparent affinity constant toward As determined by using apparent adsorption isotherms ranged from 7.90 x 10(3) to 3.31 X 10(4) mol(-1) dm(3). (C) 2008 Wiley Periodicals, Inc. J Appl Polym Sci 110: 2826-2832, 2008
引用
收藏
页码：2826 / 2832
页数：7
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