Use of PCR primers derived from a putative transcriptional regulator gene for species-specific determination of Listeria monocytogenes

被引：66

作者：

Liu, DY ^{[1
]}

Ainsworth, AJ ^{[1
]}

Austin, FW ^{[1
]}

Lawrence, ML ^{[1
]}

机构：

[1] Mississippi State Univ, Coll Vet Med, Dept Pathobiol & Populat Med, Mississippi State, MS 39762 USA

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2004年 / 91卷 / 03期

关键词：

Listeria monocytogenes; PCR; transcriptional regulator; virulence;

D O I：

10.1016/j.ijfoodmicro.2003.07.004

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Listeria monocytogenes is an opportunistic bacterial pathogen that has accounted for an important portion of human foodbome diseases worldwide. In this study, through comparative analysis of L. innoctia and L. monocytogenes genomic sequences. we selected a L. monocytogenes specific gene (lmo0733) that has the potential for specific detection of L. monocytogenes. Using PCR primers (lmo0733F and lmo0733R) derived from this gene, a specific fragment of 453 bp was amplified only from genomic DNA of L. monocytogenes strains. PCR products from other Listeria species as well as other Gram-positive and -negative species were not detectable, confirming the specificity of this assay. Thus, the PCR test employing primers lmo0733F and lmo0733R represents an additional tool in the diagnostic arsenal for rapid, sensitive and specific detection and identification of human infections due to L. monocytogenes. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：297 / 304

页数：8

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