LncRNA MAFG-AS1 Accelerates Cell Migration, Invasion and Aerobic Glycolysis of Esophageal Squamous Cell Carcinoma Cells via miR-765/PDX1 Axis

被引：21
|
作者：
Qian, Cui-juan [1 ]
Xu, Zhu-rong [1 ]
Chen, Lu-yan [1 ]
Wang, Yi-chao [2 ]
Yao, Jun [1 ]
机构：
[1] Taizhou Univ, Inst Tumor, Sch Med, Taizhou 318000, Zhejiang, Peoples R China
[2] Taizhou Univ Hosp, Taizhou Cent Hosp, Dept Lab Med, Taizhou 318000, Zhejiang, Peoples R China
来源：
CANCER MANAGEMENT AND RESEARCH | 2020年 / 12卷
基金：
中国国家自然科学基金;
关键词：
ESCC; MAFG-AS1; aerobic glycolysis; miR-765; PDX1; PROGRESSION; PROMOTES; CANCER;
D O I：
10.2147/CMAR.S262075
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background: LncRNA dysregulation is implicated in esophageal squamous cell carcinoma (ESCC) progression; However, the precise role and function of lncRNA MAFG-AS1 in ESCC remains unknown. Materials and Methods: Expressions of MAFG-AS1, miR-765, PDX1, GLUT1 and LDHA were detected via qRT-PCR or/and Western blot in ESCC tissues and cell lines. CCK-8, transwell and glycolysis assays were used to investigate the effects of MAFG-AS1 on ESCC cell proliferation, migration, invasion and aerobic glycolysis after knockdown or overexpression of MAFG-AS1, and bioinformatics analyses, RNA pull-down and dual luciferase reporter systems were applied to investigate the interaction between MAFG-AS1, miR-765 and PDX1. Results: MAFG-AS1 was significantly up-modulated in ESCC tissues and cell lines. MAFG-AS1 significantly accelerated ESCC cell proliferation, migration, invasion and aerobic glycolysis. MAFG-AS1 competitively adsorbed miR-765, while miR-765 negatively modulated the expression of PDX1. miR-765 and PDX1 participated in the promotive effects of MAFG-AS1 on cell migration, invasion and aerobic glycolysis in ESCC cells. Conclusion: Our research indicates that the MAFG-AS1/miR-765/PDX1 axis accelerates ESCC cell proliferation, migration, invasion and aerobic glycolysis.
引用
收藏
页码：6895 / 6908
页数：14
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