A glacio-fluvial aquifer located at Wurtsmith Air Force Base, Michigan, has been contaminated with JP-4 fuel hydrocarbons released by the crash of a tanker aircraft in October of 1988. A comprehensive analysis of the inorganic and organic geochemical constituents and geomicrobiological markers has documented the occurrence of in situ biodegradation of hydrocarbons in the aquifer. Concentration profiles of aromatic hydrocarbons, aromatic acids, and phospholipid ester-Iinked fatty acids (PLFA) in aquifer solids suggest microbially mediated degradation of hydrocarbons and production of aromatic acid metabolites. Microbial community structure as indicated by the PLFA patterns shows an absence of polyunsaturated fatty acids characteristic of microeukaryotes and high proportions of C-12-C-20 fatty acids typical of bacteria. Contamination increased microbial biomass by one order of magnitude and shifted the community to a more anaerobic bacterial consortium.