Fluorescence study on the interaction between apoCopC and cupric

被引：18
|
作者：
Pang, EG [1 ]
Zhao, YQ [1 ]
Yang, BS [1 ]
机构：
[1] Shanxi Univ, Inst Mol Sci, Chem Biol & Mol Engn Lab, Minist Educ, Taiyuan 030006, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2005年 / 50卷 / 20期
基金：
中国国家自然科学基金;
关键词：
cupric; apoCopC; fluorescence;
D O I：
10.1360/982005-321
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The interaction between apoCopC and cupric was investigated by fluorescence spectra, in phosphate (20 mmol/L) buffer at pH 6.0. Results suggest that the environment is measured to be hydrophobic completely around tryptophan (83). At the same time, apoCopC fluorescence at 320 urn was significantly quenched with the addition of cupric and the 1:1 stoichiometric ratio of apoCopC to cupric was confirmed by fluorescence. In addition, the conditional binding constants were calculated to be KCu-Cope = (1.8 +/- 0.58) x 10(13) mol(-1) L on the basis of the results of fluorescence titration curves. The apoCopC has the ability to bind specifically cupric ion.
引用
收藏
页码：2302 / 2305
页数：4
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