SPR Fiber Biosensor for Organic Molecules Detection

Rapid biomolecule detection is absolutely necessary procedure for disease diagnosis, food protection and environment screening. The rapid fiber biosensor for biomolecule detection based on surface plasmon resonance is proposed. Surface plasmon resonance (SPR) is widely-used technique, based on interaction of the electrical field energy of the photon and the free electron in the gold surface. The incoming photon is absorbed and the energy is transferred to the electrons, which convert into electromagnetic wave on the interface. By this approach, the most of biomolecules (proteins, lipids, DNA, carbohydrates) is possible to detect. Firstly, the fiber was tapered and the fiber surface was coated by gold nanoparticles. Two methods of coating were described and compared. Function of biosensor was verified by biotin-avidin bond with fluorescence labelling.