With the emergence of entrepreneurial society, various forms of entrepreneurships were getting people's attention. The entire business process will be subject to the initiative of entrepreneurs, so people now pay more attention to personality traits of the entrepreneurs. As for the female entrepreneurs, their character qualities are often considered to be one of the most important factors in entrepreneurial activities. This paper was started from the point of entrepreneurial opportunities, and focused on whether female entrepreneurs in terms of entrepreneurial opportunity identification are at a disadvantage, then pointed to the concept of "gender differences in entrepreneurial opportunity" and its influencing factors. Finally, we applied to the case study method to analyze gender differences in opportunities and made some useful suggestions.