The Control of Arabidopsis thaliana Growth by Cell Proliferation and Endoreplication Requires the F-Box Protein FBL17

被引:51
|
作者
Noir, Sandra [1 ]
Marrocco, Katia [2 ]
Masoud, Kinda [1 ]
Thomann, Alexis [1 ]
Gusti, Andi [1 ]
Bitrian, Marta [1 ]
Schnittger, Arp [1 ]
Genschik, Pascal [1 ,2 ]
机构
[1] Univ Strasbourg, Unite Propre Rech 2357, Inst Biol Mol Plantes, CNRS, F-67084 Strasbourg, France
[2] Inst Biol Integrat Plantes, Unite Biochim & Physiol Mol Plantes, F-34060 Montpellier, France
来源
PLANT CELL | 2015年 / 27卷 / 05期
关键词
DEPENDENT KINASE INHIBITORS; UBIQUITIN LIGASE COMPLEX; DNA-DAMAGE RESPONSE; PLANT DEVELOPMENT; TRANSCRIPTION FACTOR; CDK INHIBITORS; CYCLE CONTROL; DEGRADATION; ENDOREDUPLICATION; ROOT;
D O I
10.1105/tpc.114.135301
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A key step of the cell cycle is the entry into the DNA replication phase that typically commits cells to divide. However, little is known about the molecular mechanisms regulating this transition in plants. Here, we investigated the function of FBL17 (F BOX-LIKE17), an Arabidopsis thaliana F-box protein previously shown to govern the progression through the second mitosis during pollen development. Our work reveals that FBL17 function is not restricted to gametogenesis. FBL17 transcripts accumulate in both proliferating and postmitotic cell types of Arabidopsis plants. Loss of FBL17 function drastically reduces plant growth by altering cell division activity in both shoot and root apical meristems. In fbl17 mutant plants, DNA replication is severely impaired and endoreplication is fully suppressed. At the molecular level, lack of FBL17 increases the stability of the CDK (CYCLIN-DEPENDENT KINASE) inhibitor KIP-RELATED PROTEIN2 known to switch off CDKA; 1 kinase activity. Despite the strong inhibition of cell proliferation in fbl17, some cells are still able to enter S phase and eventually to divide, but they exhibit a strong DNA damage response and often missegregate chromosomes. Altogether, these data indicate that the F-box protein FBL17 acts as a master cell cycle regulator during the diploid sporophyte phase of the plant.
引用
收藏
页码:1461 / 1476
页数:16
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