The rotary axis is the basis for rotational motion. Its motion errors have critical effects on the accuracy of the related equipment, such as a five-axis computer numerical control machine tool. There are several difficult problems in the implementation of high-precision and fast measurement of the multi-degree-of-freedom motion errors of a rotary axis. In this paper, a novel method for the simultaneous measurement of five-degree-of-freedom motion errors of a rotary axis is proposed, which uses a single-mode fiber-coupled laser with a full-circle measuring range. It has the advantages of high efficiency, low cost, and it requires no decoupling calculation. An experimental system was built and a series of experiments were performed. The standard deviation of stability for 60 min of the five-degree-of-freedom measurement is 0.05 arcsec, 0.06 arcsec, 0.04 mu m, 0.03 mu m, and 0.19 arcsec, respectively. The repeatability deviation of measuring an indexing table is +/- 3.4 arcsec, +/- 4.6 arcsec, +/- 2.6 mu m, +/- 2.4 mu m, and +/- 3.2 arcsec. The maximum deviation of comparison is 3.9 arcsec and 3.2 arcsec. These results demonstrate the effectiveness of the proposed method; thus, a new approach of simultaneous measurement of the multi-degree-of-freedom motion errors of a rotary axis is provided. (c) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement