Two-photon-induced fluorescence from the phycoerythrin protein

被引：24
|
作者：
Chen, ZP
Kaplan, DL
Yang, K
Kumar, J
Marx, KA
Tripathy, SK
机构：
[1] USA, NATICK RES DEV & ENGN CTR, DIV BIOTECHNOL, NATICK, MA 01760 USA
[2] UNIV LOWELL, DEPT CHEM, LOWELL, MA 01854 USA
[3] UNIV LOWELL, DEPT PHYS, LOWELL, MA 01854 USA
来源：
APPLIED OPTICS | 1997年 / 36卷 / 07期
关键词：
phycobiliprotein; two-photon excitation; fluorescence; biosensors;
D O I：
10.1364/AO.36.001655
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Two-photon-induced fluorescence is observed from the photodynamic phycobiliprotein phycoerythrin. Temporal, spectral, and intensity-dependent properties of the two-photon-induced fluorescence emission from phycoerythrin excited by a 1.06-mu m laser beam are reported. The measured two-photon absorption cross section of phycoerythrin is an order of magnitude larger than that of Rhodamine 6G. The potential applications of phycobiliproteins for two-photon-induced fluorescence for microscopy of three-dimensional biological samples and three-dimensional optical memory are discussed. (C) 1997 Optical Society of America.
引用
收藏
页码：1655 / 1659
页数：5
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