Cost Effective Production of Poly-β-Hydroxybutyrate by Bacillus subtilis NG05 Using Sugar Industry Waste Water

被引:19
|
作者
Singh, Gulab [1 ]
Kumari, Anish [1 ]
Mittal, Arpana [1 ]
Goel, Varsha [1 ]
Yadav, Anita [2 ]
Aggarwal, Neeraj Kumar [1 ]
机构
[1] Kurukshetra Univ, Dept Microbiol, Kurukshetra 136119, Haryana, India
[2] Kurukshetra Univ, Dept Biotechnol, Kurukshetra 136119, Haryana, India
关键词
Sugar industry waste water; Poly beta-hydroxybutyrate production; Bacillus subtilis; Nile Blue A; Molecular characterization; GROWTH; IDENTIFICATION; OPTIMIZATION; SUBSTRATE; SYNTHASE;
D O I
10.1007/s10924-012-0533-3
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The present study describes the treatment of sugar industry waste water and its use as a potential low cost substrate for production of bioplastic by Bacillus subtilis NG05. The B. subtilis NG05 grow at the rate of 0.14 g h(-1) L-1 of production media used and accumulate the 50.1 % of poly beta-hydroxybutyrate (PHB). The phase contrast microscopy revealed the presence of PHB granules in B. subtilis NG05 which was further confirmed by Fourier transform infrared spectroscopy and H-1-nuclear magnetic resonance. The polymer was further analysed by differential scanning calorimetry. PHB production yield was achieved up to 4.991 g L-1 with Sugar industry waste water as sole nutrient source. Thus the process provided dual benefits of conversion of a waste material into value added product, PHB and waste management.
引用
收藏
页码:441 / 449
页数:9
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