Ganoderic Acid Me Inhibits Tumor Invasion Through Down-Regulating Matrix Metalloproteinases 2/9 Gene Expression

被引:93
|
作者
Chen, Nian-Hong [2 ]
Liu, Jian-Wen [2 ]
Zhong, Jian-Jiang [1 ]
机构
[1] Shanghai Jiao Tong Univ, Key Lab Microbial Metab, Minist Educ, Coll Life Sci & Biotechnol, Shanghai 200240, Peoples R China
[2] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
关键词
ganoderic acid Me (GA-Me); invasion; matrix metalloproteinases 2/9 (MMP2/9);
D O I
10.1254/jphs.SC0080019
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The effect of ganoderic acid Me (GA-Me), which was purified from the fermentation mycelia of the traditional Chinese medicinal mushroom Ganoderma lucidum as reported (Tang W, Gu TY, Zhong JJ. Biochem Eng J. 2006;32:205-210), on anti-invasion was investigated. Wound healing assay indicated that GA-Me inhibited cell migration of 95-D, a human highly metastatic lung tumor cell line, in dose- and time-dependent manners. Results of cell aggregation and adhesion assays showed that GA-Me promoted cell homotypic aggregation and inhibited cell adherence to extracellular matrix (ECM). In addition, GA-Me suppressed matrix metalloproteinases 2/9 (MMP2/9) gene expressions at both mRNA and protein levels in 95-D cells according to qRT-PCR and Western blotting, respectively. The results demonstrated that GA-Me effectively inhibited tumor invasion, and it might act as a new MMP2/9 inhibitor for anti-metastasis treatment of carcinoma cells.
引用
收藏
页码:212 / 216
页数:5
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